Day buses run from 4:30 a.m. till midnight and evening buses run midnight till 4:30 a.m. The main benefit of Prague city buses is that they run where the tram and metro do not. Over 300 milion passengers use the buses yearly and there are over 200 lines to choose from. The two most frequently used Prague bus stations are Florenc and Roztyly. Florenc is the international bus station and where you will most likely need to go if you are traveling by bus to a location outside of Prague.